Modulation of LXR signaling altered the dynamic activity of human colon adenocarcinoma cancer stem cells in vitro

Abstract Background The expansion and metastasis of colorectal cancers are closely associated with the dynamic growth cancer stem cells (CSCs). This study aimed to explore possible effect LXR (a regulator glycolysis lipid hemostasis) in tumorgenicity human CD133 cells. Methods Human HT-29 + were enriched by MACS incubated agonist (T0901317) antagonist (SR9243) for 72 h. Cell survival was evaluated using MTT assay flow cytometric analysis Annexin-V. proliferation rate measured monitoring Ki-67 positive IF imaging. modulation studied activity all factors related ABC transporters real-time PCR western blotting. Protein levels metabolic enzymes such as PFKFB3, GSK3?, FASN, SCD also investigated upon treatment CSCs modulators. migration monitored after being exposed scratch Transwell insert assays. efflux capacity hypo-osmotic conditions. intracellular content reactive oxygen species DCFH-DA staining. Results Data showed incubation T0901317 SR9243 reduced viability a dose-dependent manner compared control group. activation up-regulated expression protein (ABCA1, ABCG5, ABCG8) non-treated (p < 0.05). Despite these effects, suppressed proliferation, clonogenicity, cells, increased fragility We that inhibited clonogenicity through down-regulating Intracellular ROS inhibition Calling attention, both compounds induced apoptotic changes Conclusions regulation can be considered selective targeting survival, metabolism, tumorigenesis patients advanced cancers.